LOCUS       sequence-18724-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to 
            be recombined out of the construct, activating Dnmt1 shRNA 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L,
            Jaenisch R, Jacks T
  TITLE     Cre-lox-regulated conditional RNA interference from transgenes.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
  PUBMED    15240889
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jul 13. 101(28):10380-5."
COMMENT     Sequence Label: pSico Dnmt1
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(12..45)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     110..126
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    201..789
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(254..274)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(672..683)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(792..808)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtagcggccg cataacttcg tatagtataa attatacgaa gttataagcc ttgtttgagt
       61 gtgtgaggga gaaattcaag agatttctcc ctcacacact cttttttctc gaggtcgacg
      121 gtatcgataa gctcgcttca cgagatcatg tttaagggtt ccggttccac taggtacaat
      181 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
      241 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
      301 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
      361 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
      421 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
      481 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
      541 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
      601 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
      661 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
      721 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
      781 cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca
      841 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
      901 gaggtgggtt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
      961 tgtagatctt agccactttt taaaagaaaa gggggactgg a
//
