LOCUS       sequence-187351-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human CAMK4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: CAMK4 gRNA (BRDN0001146440)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        77..152
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    208..325
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggagc
       61 ataccgcagt accctggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      121 atcaacttga aaaagtggca ccgagtcggt gcttttttaa gcttggcgta actagatctt
      181 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
      241 tgcaggggaa agaatagtan acataatagc aacagacata caaactaaag aattacaaaa
      301 acaaattaca aaaattcaaa attttcgggt ttattacang gacagcagan atccactttg
      361 gcgccggctc gagggggccc gggtgcaaag atggataaag ttttnaacag anaggaatcn
      421 ttgcagctaa tggaccttct angtcttgaa angagtggga nttggctccg gtgcccgtca
      481 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tccggcnant
      541 tgatccggtg cctanagaag gtgcgcgcgg
//
