LOCUS       sequence-187361-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PRKD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PRKD1 gRNA (BRDN0001144834)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..109
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        189..264
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    320..437
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cacacnnnat attagtaccc ccttnnnggg gcgnngaaag tnataatttc ttggggagnt
       61 tgcngccttt naaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
      121 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgca ttaatggtca
      181 cttcgccagt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      241 gaaaaagtgg caccgagtcg gtgctttttt aagcttggcg taactagatc ttgagacaaa
      301 tggcagtatt catccacaat tttaaaanaa aaggggggat tggggggtac agtgcagggg
      361 aaagaatagt agacataata gcaacagaca tacaaactaa ngaattacaa aaacaaatta
      421 caaaaattca aaattttcng gtttattaca gggacagcag agatccactt tggcgccggc
      481 tcgagggggc ccgggtgcaa agatggataa agttcttaaa caganaggaa nctttgcagc
      541 taatggacct tctaggtctt gaaaggagtg
//
