LOCUS       sequence-187377-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human MYLK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: MYLK2 gRNA (BRDN0001148218)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..44
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        124..199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    255..372
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
       61 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgtggctgg cctgggatcc
      121 gtggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      181 agtggcaccg agtcggtgct tttttaagct tggcgtaact agatcttgag acaaatggca
      241 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      301 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      361 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag
      421 ggggcccggg tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
      481 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
      541 acatcgccca cagtccccga gaagttgggg
//
