LOCUS       sequence-187385-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human FYN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: FYN gRNA (BRDN0001147012)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        138..213
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    269..386
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
       61 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgttg
      121 tcctttggaa acccaaggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      181 tatcaacttg aaaaagtggc accgagtcgg tgctttttta agcttggcgt aactagatct
      241 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
      301 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
      361 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
      421 ggcgccggct cgagggggcc cgggtgcaaa gatggataaa gttttaaaca gagaggaatc
      481 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      541 agtgggcaga gcgcacatcg cccacagtcc
//
