LOCUS       sequence-187451-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PKMYT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PKMYT1 gRNA (BRDN0001146750)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        141..216
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    272..389
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
       61 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      121 agcagcggat gtgttcaggt gttttagagc tagaaatagc aagttaaaat aaggctagtc
      181 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttaagcttgg cgtaactaga
      241 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
      301 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
      361 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
      421 tttggcgccg gctcgagggg gcccgggtgc aaagatggat aaagttttaa acagagagga
      481 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
      541 gtcagtgggc agagcgcaca tcgcccacag
//
