LOCUS       sequence-18748-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Nakamura K, Johnson GL
  TITLE     PB1 domains of MEKK2 and MEKK3 interact with the MEK5 PB1 domain for
            activation of the ERK5 pathway.
  JOURNAL   J Biol Chem. 2003 Sep 26. 278(39):36989-92.
  PUBMED    12912994
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Sep 26. 278(39):36989-92."
COMMENT     Sequence Label: pMSCV MEKK2 PB1
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..61
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(388..411)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tctagggcgc cggaattaga tctctcgagc caccatggat tacaaggatg acgacgataa
       61 gccttcatca ccaaaaaaac agaatgatgt tcgagtcaaa tttgaacata gaggagaaaa
      121 aaggatcctg caggttacta gaccagttaa actagaagac ctgagatcta agtctaagat
      181 cgcctttggg cagtctatgg atctacacta taccaacaat gagttggtaa ttccgttaac
      241 tacccaagat gacttggaca aagctgtgga actgctggat cgcagtattc acatgaagag
      301 tctcaagata ttacttgtag taaatgggag tacacaggct tagaattcta ccgggtaggg
      361 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      421 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      481 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      541 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
      601 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
      661 ggccaatagc agctttgctc cttcgctttc tg
//
