LOCUS       sequence-18749-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Nakamura K, Johnson GL
  TITLE     PB1 domains of MEKK2 and MEKK3 interact with the MEK5 PB1 domain for
            activation of the ERK5 pathway.
  JOURNAL   J Biol Chem. 2003 Sep 26. 278(39):36989-92.
  PUBMED    12912994
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Sep 26. 278(39):36989-92."
COMMENT     Sequence Label: pMSCV MEKK3 PB1
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..73
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(400..423)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta gatctctcga gccaccatgg attacaagga
       61 tgacgacgat aagacaggtc acccaaacag gcagagtgac gtcagaatca agtttgaaca
      121 caatggggag agacgaatta tagcattcag ctggcctgtg agatacgaag atgtggagca
      181 caaggtgaca acagtctttg ggcagcctct tgatttgcat tatatgaata atgagctctc
      241 catcctgttg aaaaaccaag atgatctcga taaagccatt gacattttgg atagaagctc
      301 aagtatgaaa agccttagga tactactgtt atcccaagac agaaaccata cttagaattc
      361 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
      421 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
      481 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
      541 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
      601 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
      661 ctttggggca gcggccaata gcagctttgc tccttcgctt tctg
//
