LOCUS       sequence-187617-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human CSNK1G3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: CSNK1G3 gRNA (BRDN0001144972)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        155..230
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tagaaagtaa taatttcttg ggtagtttgc ccctttanng ggatgtttta aaatggacta
       61 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttanccc nnaaggggaa
      121 aggacgaaac accgaacttc ttaataggac gaccgtttta gagctagaaa tagcaagtta
      181 aaataaggct agtccgttat caacttgaaa aagtggnacc gagtcggtgc ttttttaagc
      241 ttggngtaac tagatcttga gacaaatggn agtattcatc cacaatttta aaagaaaagg
      301 ggggattggg gggtacagtg naggggaaag aatagtagan ataatagnaa caganataca
      361 aactaaagaa ttanaaaaan aaattanaaa aattnaaaat tttngggttt attanaggga
      421 cagnagagat ccantttggn gncggntcga gggggncngg gtgnaaagat ggataaagtt
      481 ttaaanagag aggaatcttt gnagntaatg gannttntag gtnttgaaag gagtgggaat
      541 tggntnnggt gnnngtnagt gggnagagng
//
