LOCUS       sequence-18764-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Zaehres H, Lensch MW, Daheron L, Stewart SA, Itskovitz-Eldor J, 
            Daley GQ
  TITLE     High-efficiency RNA interference in human embryonic stem cells.
  JOURNAL   Stem Cells. 2005 Mar . 23(3):299-305.
  PUBMED    15749924
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2005 Mar . 23(3):299-305."
COMMENT     Sequence Label: LL - hOCT4i -2
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..73
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     58..74
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(116..149)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        265..568
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        569..772
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     723..743
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      811..820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(863..884)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttggatgtgg tccgagtgtg gttcaagaga ccacactcgg accacatcgt tttttctcga
       61 ggtcgacggt atcgataagc tcgcttcacg agattccagc aggtcgaggg acctaataac
      121 ttcgtatagc atacattata cgaagttata ttaagggttc caagcttaag cggccgcgtg
      181 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
      241 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      301 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      361 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      421 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      481 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      541 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      601 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      661 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      721 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
      781 gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
      841 ttcaccgggg tggtgcccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      901 cagc
//
