LOCUS       sequence-187737-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PAN3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PAN3 gRNA (BRDN0001146176)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        24..99
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    155..272
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccggcatggt gttggtcgac atggttttag agctagaaat agcaagttaa aataaggcta
       61 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct tggcgtaact
      121 agatcttgag acanatggca gtattcntcc acaattttaa aagaaaaggg gggattgggg
      181 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaangaat
      241 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
      301 cactttggcg ccggctcgag ggggcccggg tgcaaagatg gataaagttt tacacagaga
      361 ggaatctttg cagctaatgg accttctagg tcttgaanag gagtgggaat tggctccggt
      421 gcccgtcagg tgggcanatc ncacctcgcc cacantnccc cnanaagttg ggggnagggg
      481 tcggcnattg atccngtgnc tagagaaggt ggcncgggng aaactgngaa aagtgatgtc
      541 gtgtccngnc ctcccccttt ttncccgagg
//
