LOCUS       sequence-18778-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Wiznerowicz M, Trono D
  TITLE     Conditional suppression of cellular genes: lentivirus 
            vector-mediated drug-inducible RNA interference.
  JOURNAL   J Virol. 2003 Aug . 77(16):8957-61.
  PUBMED    12885912
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2003 Aug . 77(16):8957-61."
COMMENT     Sequence Label: pLVTH-sip53
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(119..152)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             172..352
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(377..395)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(378..395)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ggatcttata agttctgtat gagaccacag atccccgact ccagtggtaa tctacttcaa
       61 gagagtagat taccactgga gtctttttgg aaaagcttat cgataccgtc gcatgggaat
      121 aacttcgtat agcatacatt atacgaagtt atgctgcttt ttgcttgtac tgggtctctc
      181 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      241 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      301 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
      361 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
      421 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
      481 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
      541 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
      601 tcagcagggt catcgctagc cagatcctct acgccggacg catcttggcc ggcatcaccg
      661 gcgccacagg tggggttgct ggcgcctata tc
//
