LOCUS       sequence-187781-         568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human ITK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: ITK gRNA (BRDN0001147252)
FEATURES             Location/Qualifiers
     source          1..568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        157..232
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             526..538
                     /estimated_length=13
     gap             540..550
                     /estimated_length=11
ORIGIN
        1 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
       61 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      121 aaaggacgaa acaccgggaa ggggctatgt cagaaggttt tagagctaga aatagcaagt
      181 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttaa
      241 gcttggcgta actagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
      301 ggggggattg gggggtacag tgcangggaa agaatantan acataatagc aacagacata
      361 caaactaaag aattacnaaa acaaattacn nanattcaaa atttncgggt tcantacngg
      421 gacancnnan atccnctttg gcgccggcnc nnnggngccn ggnnncnnnn nnggnnnaan
      481 nnnnnnncnn annnancccc ngcnncnnan gnnnnnnncc nnnncnnnnn nnnnnnnngn
      541 nnnnnnnnnn cnnnncncnn nnnnnncc
//
