LOCUS       sequence-18785-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPT-GFP
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(80..291)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    348..465
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgac cggtagcgct aggacgcgta agcttaggcc
       61 tggatccgcg tcacgacacc tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca
      121 cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg cggagccagt
      181 acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg gttcaattgc
      241 cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc cactgacggg
      301 caccggagcc atcgatcaag cttctcgagg ctagtctcgt gatcgataaa attttgaatt
      361 tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt
      421 ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaagttaa ccgataccgt
      481 cgagatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct
      541 attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt
      601 gaatatccct gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
      661 accaagcctc ctactatcat tatgaataat ttta
//
