LOCUS       sequence-18789-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Wiznerowicz M, Trono D
  TITLE     Conditional suppression of cellular genes: lentivirus 
            vector-mediated drug-inducible RNA interference.
  JOURNAL   J Virol. 2003 Aug . 77(16):8957-61.
  PUBMED    12885912
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2003 Aug . 77(16):8957-61."
COMMENT     Sequence Label: pLVTH
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             5..184
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    204..237
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(270..484)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(330..349)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    513..531
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    555..573
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    597..615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atgctgctag agattttcac actgactaaa agggtctgag ggatctctag ttaccagagt
       61 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
      121 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
      181 acccagtaca agcaaaaagc agcataactt cgtataatgt atgctatacg aagttattcc
      241 catgcgacgg tatcgataag cttagatctg tggtctcata cagaacttat aagattccca
      301 aatccaaaga catttcacgt ttatggtgat ttcccagaac acatagcgac atgcaaatat
      361 tgcagggcgc cactcccctg tccctcacag ccatcttcct gccagggcgc acgcgcgctg
      421 ggtgttcccg cctagtgaca ctgggcccgc gattccttgg agcgggttga tgacgtcagc
      481 gttcgaattc ctgcagcccc gactttcact tttctctatc actgataggg agtggtaaac
      541 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
      601 tatcactgat agggagtgg
//
