LOCUS       sequence-18791-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pLOX-CWgfp
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(127..151)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(148..351)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(177..197)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        352..655
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgac cggtagcgct aggacgcgta agcttaggcc
       61 tggatcctct agctagagtc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct
      121 ccaggcgatc tgacggttca ctaaacgagc tctgcttata tagacctccc accgtacacg
      181 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
      241 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
      301 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
      361 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
      421 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
      481 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
      541 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
      601 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
      661 ctcccaagct tatcgatacc gtcgagatcc gttcactaat cgaatggatc tgtctctgtc
      721 tctctctccc cttcttcttc t
//
