LOCUS       sequence-18798-e         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pHRgfpLox
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(151..184)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             204..384
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(409..427)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(410..427)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cttcgaatta attctgcagt cgacggtacc tttaagacca atgacttaca aggcagctgt
       61 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
      121 aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca ttatacgaag
      181 ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
      241 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      301 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      361 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
      421 cctaaatcgt atgtgtatga tacataatgt tatgtattaa ttgtagccgc gttctaacga
      481 caatatgtac aagcctaatt gtgtagcatc tggcttactg aaacagaccc tatcatctct
      541 ctcgtaaact gccgtcagaa tcggtttggt tggacgaacc ttctgaattt ctgg
//
