LOCUS       sequence-18799-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Salmon P, Kindler V, Ducrey O, Chapuis B, Zubler RH, Trono D
  TITLE     High-level transgene expression in human hematopoietic progenitors 
            and differentiated blood lineages after transduction with improved 
            lentiviral vectors.
  JOURNAL   Blood. 2000 Nov 15. 96(10):3392-8.
  PUBMED    11071633
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2000
            Nov 15. 96(10):3392-8."
COMMENT     Sequence Label: pLOX-EWgfp
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          108..311
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(131..151)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tcctcgccct tgctcaccat ggtggcgacc ggtagcgcta ggacgcgtaa gcttaggcct
       61 ggatcccggg cccgcggtac cgtcgactgc agaattcctc acgacacctg aaatggaaga
      121 aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa ctcaaaaagg
      181 gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt ctccacccac
      241 tcagtgtggg gaaactccat cgcataaaac ccctcccccc aacctaaaga cgacgtactc
      301 caaaagctcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt ggagtcacat
      361 gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc acccgcccgc
      421 tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg aagcggccat
      481 ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg cggggcgata
      541 cacggcggcg cgaggccagg caccagagca ggccggccag cttgagacta cccccgtccg
      601 attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg ggacgcacgg
      661 gccccgtcgc cgcccg
//
