LOCUS       sequence-188137-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PFKFB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PFKFB1 gRNA (BRDN0001148427)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        120..195
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    251..368
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
       61 tcttggcttt atatatcttg tggaaaggac gaaacaccga tggtgggttt accagctcgg
      121 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      181 gcaccgagtc ggtgcttttt taagcttggc gtaactagat cttgagacaa atggcagtat
      241 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      301 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
      361 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg
      421 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
      481 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      541 cgcccacagt ccccgagaag ttggggggag
//
