LOCUS       sequence-188153-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human ARSG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: ARSG gRNA (BRDN0001148698)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        113..188
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    244..361
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
       61 tttatatatc ttgtggaaag gacgaaacac cgaggtaagg gcacgtgcat gtgttttaga
      121 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      181 gtcggtgctt ttttaagctt ggcgtaacta gatcttgaga caaatggcag tattcatcca
      241 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      301 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcnaaattt
      361 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggt
      421 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
      481 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
      541 agtccccgag aagttggggg gaggggttcg
//
