LOCUS       sequence-18816-e         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Le Y, Miller JL, Sauer B
  TITLE     GFPcre fusion vectors with enhanced expression.
  JOURNAL   Anal Biochem. 1999 Jun 1. 270(2):334-6.
  PUBMED    10334853
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 1999 Jun 1. 270(2):334-6."
COMMENT     Sequence Label: pBS505 EF1alpha-EGFPcre*
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          29..232
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(52..72)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggtagcgcta gagaattcct cacgacacct gaaatggaag aaaaaaactt tgaaccactg
       61 tctgaggctt gagaatgaac caagatccaa actcaaaaag ggcaaattcc aaggagaatt
      121 acatcaagtg ccaagctggc ctaacttcag tctccaccca ctcagtgtgg ggaaactcca
      181 tcgcataaaa cccctccccc caacctaaag acgacgtact ccaaaagctc gagaactaat
      241 cgaggtgcct ggacggcgcc cggtactccg tggagtcaca tgaagcgacg gctgacgacg
      301 gaaaggccct tttcctttgt gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc
      361 tccattttga gctccctgca gcagggccgg gaagcggcca tctttccgct cacgcaactg
      421 gtgccgaccg ggccagcctt gccgcccagg gcggggcgat acacggcggc gcgaggccag
      481 gcaccagagc aggccggcca gcttgagact acccccgtcc gattctcggt ggccgcgctc
      541 gcaggccccg cctcgccgaa catgtgcgct g
//
