LOCUS       sequence-18823-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Gagneten S, Le Y, Miller J, Sauer B
  TITLE     Brief expression of a GFP cre fusion gene in embryonic stem cells 
            allows rapid retrieval of site-specific genomic deletions.
  JOURNAL   Nucleic Acids Res. 1997 Aug 15. 25(16):3326-31.
  PUBMED    9241248
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 1997 Aug 15. 25(16):3326-31."
COMMENT     Sequence Label: pBS500 EF1alpha-GFPcre
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          56..259
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(79..99)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tctcctttac tcatggcact cgaccaagct atctggatcc aattcctcac gacacctgaa
       61 atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact
      121 caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct
      181 ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg
      241 acgtactcca aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg
      301 agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac
      361 ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa
      421 gcggccatct ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg
      481 gggcgataca cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc
      541 cccgtccgat tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg
      601 acgcacgggc cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt
      661 ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat
      721 tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc
      781 gcacgcggcg gcccc
//
