LOCUS       sequence-188241-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PFKFB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PFKFB2 gRNA (BRDN0001147256)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        100..175
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    231..348
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
       61 tggaaaggac gaaacaccga aagcgagttc aatctcttgg ttttagagct agaaatagca
      121 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      181 taagcttggc gtaactagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
      241 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      301 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      361 agggcacagc agagatccac tttggcgccg gctcnagggg gcccgggtgc aaagatggat
      421 aaagttttaa acagagagga atctttgcca gctaatgnac cttcnaggtc ttgaaaggag
      481 tggganttgg ctccgggtgc cccgtcagtg ggcagagcnc acatcgcccc acagtccccg
      541 agaagttggg ggggaggggg tccngccatt
//
