LOCUS       sequence-188261-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human TP53RK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: TP53RK gRNA (BRDN0001145338)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        182..257
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    313..430
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atattagcnc cccctttaag gggacgctag anagtaataa tttcttgggt agtttgccnt
       61 tttaaaatta tgttttaaaa tggactatca tatgcntacc gtaacttgaa agtatttcga
      121 tttcttggct ttatatatct tgtggaaagg acgaaacacc ggagactgaa aaaactcccc
      181 agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      241 tggcaccgag tcggtgcttt tttaagcttg gcgtaactag atcttgagac aaatggcagt
      301 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      361 agtagacata atagcaacag acatacaaac tnaagaatta caaaaacaaa ttacaaaaat
      421 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
      481 ggcccgggtg caaagatgga taaagtttta aacagagagg aatcttntgc agctaangga
      541 ccttctaggt cttgaaagga gtgggaattt
//
