LOCUS       sequence-18827-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Sells MA, Knaus UG, Bagrodia S, Ambrose DM, Bokoch GM, Chernoff J
  TITLE     Human p21-activated kinase (Pak1) regulates actin organization in 
            mammalian cells.
  JOURNAL   Curr Biol. 1997 Mar 1. 7(3):202-10.
  PUBMED    9395435
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            1997 Mar 1. 7(3):202-10."
COMMENT     Sequence Label: pCMV6M-Pak1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..101
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(646..663)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtgaccgtca gaattaattc agatctggta ccacgcgtat cgataagctt gcatgcctgc
       61 aggtcgacat ggaacagaaa ctcatctctg aagaggatct gtctagagga tccatgtcaa
      121 ataacggcct agacattcaa gacaaacccc cagcccctcc gatgagaaat accagcacta
      181 tgattggagc cggcagcaaa gatgctggaa ccctaaacca tggttctaaa cctctgcctc
      241 caaacccaga ggagaagaaa aagaaggacc gattttaccg atccatttta cctggagata
      301 aaacaaataa aaagaaagag aaagagcggc cagagatttc tctcccttca gattttgaac
      361 acacaattca tgtcggtttt gatgctgtca caggggagtt tacgggaatg ccagagcagt
      421 gggcccgctt gcttcagaca tcaaatatca ctaagtcgga gcagaagaaa aacccgcagg
      481 ctgttctgga tgtgttggag ttttacaact cgaagaagac atccaacagc cagaaataca
      541 tgagctttac agataagtca gctgaggatt acaattcttc taatgccttg aatgtgaagg
      601 ctgtgtctga gactcctgca gtgccaccag tttcagaaga tgaggatgat gatgatgatg
      661 atgctacccc accaccagtg attgctccac gcccagagca cacaaaatct gtatacacac
      721 ggtctgtgat tgaaccactt cctgtcactc caactcggga cgtggctaca tctcccattt
      781 cacctactga aaataacacc actccaccag atgctttgac ccggaatact gagaagcaga
      841 agaagaagcc taaaatgtct gatgaggaga tcttggagaa ttacgaagca tagtgagtgg
//
