LOCUS       sequence-18832-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Galisteo ML, Chernoff J, Su YC, Skolnik EY, Schlessinger J
  TITLE     The adaptor protein Nck links receptor tyrosine kinases with the 
            serine-threonine kinase Pak1.
  JOURNAL   J Biol Chem. 1996 Aug 30. 271(35):20997-1000.
  PUBMED    8798379
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1996 Aug 30. 271(35):20997-1000."
COMMENT     Sequence Label: pCMV6M-Pak1 P13A
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..112
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(657..674)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agctcgttta gtgaaccgtc agaattaatt cagatctgct agcacgcgta tcgataagct
       61 tgcatgcctg caggtcgaca tggaacagaa actcatctct gaagaggatc tgtctagagg
      121 atccatgtca aataacggcc tagacattca agacaagccg gcagcccctc cgatgagaaa
      181 taccagcact atgattggag ccggcagcaa agatgctgga accctaaacc atggttctaa
      241 acctctgcct ccaaacccag aggagaagaa aaagaaggac cgattttacc gatccatttt
      301 acctggagat aaaacaaata aaaagaaaga gaaagagcgg ccagagattt ctctcccttc
      361 agattttgaa cacacaattc atgtcggttt tgatgctgtc acaggggagt ttacgggaat
      421 gccagagcag tgggcccgct tgcttcagac atcaaatatc actaagtcgg agcagaagaa
      481 aaacccgcag gctgttctgg atgtgttgga gttttacaac tcgaagaaga catccaacag
      541 ccagaaatac atgagcttta cagataagtc agctgaggat tacaattctt ctaatgcctt
      601 gaatgtgaag gctgtgtctg agactcctgc agtgccacca gtttcagaag atgaggatga
      661 tgatgatgat gatgctaccc caccaccagt gattgctcca cgcccagagc acacaaaatc
      721 tgtatacaca cgg
//
