LOCUS       sequence-18833-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Bagrodia S, Taylor SJ, Creasy CL, Chernoff J, Cerione RA
  TITLE     Identification of a mouse p21Cdc42/Rac activated kinase.
  JOURNAL   J Biol Chem. 1995 Sep 29. 270(39):22731-7.
  PUBMED    7559398
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1995 Sep 29. 270(39):22731-7."
COMMENT     Sequence Label: pGEX-Rac1
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..23
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             27..602
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="QAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMV
                     DGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVSLICFSLVSPASFENVRAKWYPEVRHHC
                     PNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRG
                     LKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(661..683)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cggatctggt tccgcgtgga tccccgcagg ccatcaagtg tgtggtggtg ggagacggag
       61 ctgtaggtaa aacttgccta ctgatcagtt acacaaccaa tgcatttcct ggagaatata
      121 tccctactgt ctttgacaat tattctgcca atgttatggt agatggaaaa ccggtgaatc
      181 tgggcttatg ggatacagct ggacaagaag attatgacag attacgcccc ctatcctatc
      241 cgcaaacaga tgtgtcctta atttgctttt cccttgtgag tcctgcatca tttgaaaatg
      301 tccgtgcaaa gtggtatcct gaggtgcggc accactgtcc caacactccc atcatcctag
      361 tgggaactaa acttgatctt agggatgata aagacacgat cgagaaactg aaggagaaga
      421 agctgactcc catcacctat ccgcagggtc tagccatggc taaggagatt ggtgccgtaa
      481 aatacctgga gtgctcggcg ctcacacagc gaggcctcaa gacagtgttt gacgaagcga
      541 tccgagcagt cctctgcccg cctcccgtga agaagaggaa gagaaaatgc ctgctgttgt
      601 aagaattcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa
      661 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
      721 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
      781 ccagtcacgt
//
