LOCUS       sequence-188333-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human NEK7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: NEK7 gRNA (BRDN0001145539)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    228..345
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
       61 aaaggacgaa acaccgacct aaagaatgtg cagctggttt tanagctaga aatancaagt
      121 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggn gcttttttaa
      181 gcttggcgta actagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
      241 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      301 caaactaaag aattacaaaa ncaaattaca aaaattcaaa attttcgggt ttattacagg
      361 gacagcagag atccactttg gcgccggctc nagggggccc gggtgcaaag atggataaag
      421 ttttaancag agaggaatct ttgcngctaa tggacctnct aggtcttgaa aggactggga
      481 attggctccg gtgcccgtca gtgggcanag cgcacatcgc ccacantccc cganaagttg
      541 ggggggaggg gtcggcaatt gatcccggtg
//
