LOCUS       sequence-188395-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human CDC42BPB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: CDC42BPB gRNA (BRDN0001148622)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        128..203
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    259..376
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tgcantttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
       61 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgaga ggaattggtc
      121 agacgtggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      181 aaaaagtggc accgagtcgg tgctttttta agcttggcgt aactagatct tgagacaaat
      241 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      301 aagaatagta gacntaatag caacagacat acaaactaaa gaattacaaa aacaaattac
      361 aaaaattcna aattttcggg tttattacag ggacagcaga gatccncttt ggcgccggct
      421 cgagggggcc cgggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
      481 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgccccgt cagtgggcna
      541 gagcgcacat cgcccaccgt ccccgagaag
//
