LOCUS       sequence-18846-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Xiao GH, Beeser A, Chernoff J, Testa JR
  TITLE     p21-activated kinase links Rac/Cdc42 signaling to merlin.
  JOURNAL   J Biol Chem. 2002 Jan 11. 277(2):883-6.
  PUBMED    11719502
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Jan 11. 277(2):883-6."
COMMENT     Sequence Label: pGEX-Pak1 83-149
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(308..330)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(475..493)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        561..665
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatcggatct ggttccgcgt ggatcccata caattcatgt tggttttgat gctgtcacag
       61 gggagtttac ggggatgcca gaacagtggg cccgcttgct tcaaacatca aatatcacca
      121 agtcagagca gaagaaaaac ccacaggctg ttctggatgt gttggaattt tataactcca
      181 agaagacctc caatagtcag aagtacatga gttttacaga taagtcagaa ttcccgggtc
      241 gactcgagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg
      301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
      421 ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg
      481 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      541 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      601 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      661 agagtatgag tattcaacat ttccgtgtcg cccttatttc cttttttgcg gcattttgcc
      721 ttccc
//
