LOCUS       sequence-188469-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human IP6K2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: IP6K2 gRNA (BRDN0001145651)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        143..218
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    274..391
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
       61 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
      121 cgaatagcat atccattgaa aggttttaga gctagaaata gcaagttaaa ataaggctag
      181 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttaagctt ggcgtaacta
      241 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
      301 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
      361 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
      421 actttggcgc cggctcgagg gggcccgggt gcaaagatgg ataaagtttt aaacagagag
      481 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc
      541 ccgtcagtgg gcagagcgca catcgcccac
//
