LOCUS       sequence-188509-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PHKA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PHKA1 gRNA (BRDN0001148113)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        140..215
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    271..388
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
       61 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      121 taggggctag tttaggatgg ttttagagct agaaatagca agttaaaata aggctagtcc
      181 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc gtaactagat
      241 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
      301 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      361 aaaacnaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
      421 ttggcgccgg ctcgaggggg cccgggtgca aagatggata aagttttaaa cagagaggaa
      481 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
      541 tcagtgggca gagcgcacat cgcccacagt
//
