LOCUS       sequence-18851-e         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Kaczmarczyk SJ, Green JE
  TITLE     A single vector containing modified cre recombinase and LOX 
            recombination sequences for inducible tissue-specific amplification 
            of gene expression.
  JOURNAL   Nucleic Acids Res 2001 Jun 15;29(12):E56-6.
  PUBMED    11410679
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "2001-06-15"; volume: "29(12)"; pages: "E56-6"
COMMENT     Sequence Label: p231 pCMVe-betaAc-STOP-luc
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    48..81
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    106..240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(130..149)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     184..203
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggcgtcttcc atggtggctt taccaacagt accggaatgc caagcttata acttcgtata
       61 atgtatgcta tacgaagtta tcctaggagc ttacttacca tgtcagatcc agacatgata
      121 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      181 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      241 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      301 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
      361 tatacatcaa atattcctta ttaacccctt tacaaattaa aaagctaaag gtacacaatt
      421 tttgagcata gttattaata gcagacactc tatgcctgtg tggagtaaga aaaaacagta
      481 tgttatgatt ataactgtta tgcctactta taaaggttac agaatatttt tccataattt
      541 tcttgtatag cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc
      601 tattactaaa cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt
      661 cttctacctt tctc
//
