LOCUS       sequence-188517-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PHKB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PHKB gRNA (BRDN0001149204)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        126..201
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    257..374
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
       61 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgtgagt acctttgatg
      121 tcccggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      181 aaagtggcac cgagtcggtg cttttttaag cttggcgtaa ctagatcttg agacaaatgg
      241 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      301 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      361 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
      421 agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
      481 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
      541 gcacatcgcc cacagtcccc gagaagttgg
//
