LOCUS       sequence-188541-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human KSR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: KSR1 gRNA (BRDN0001147076)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        49..124
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    180..297
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tatatcttgt ggaaaggacg aaacaccggt tggagttcat tggatgcggt tttagagcta
       61 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      121 gtgctttttt aagcttggcg taactagatc ttgagacaaa tggcagtatt catccacaat
      181 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
      241 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattcn aaattttcgg
      301 gtttattaca gggcacagca nagatccact ttggcgccgg ctcnaggggg cccgggtgca
      361 aagatggata aagttttaaa canagaggaa tctttgcagc taatggacct tctaggtctt
      421 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cggcccacag
      481 tccccgaaga agttgggggg ganggggtcg ggcaattgat cccggtgcct anagaaacgt
      541 ggcncggggn tnaaactggn aaaantngat
//
