LOCUS       sequence-188557-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human TAF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: TAF1 gRNA (BRDN0001145911)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     102..121
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        201..276
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 accggtanac acaaagatat tagntactcc ccntttnngg ncgntagaaa gtaataattt
       61 cttgggtaga tttgcagcnt ttaaaattat gttttaaaat ggactatcat atgcttaccg
      121 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      181 tctggtatat ggacgctggg gttttagagc tagaaatagc aagttaaaat aaggctagtc
      241 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttaagcttgg cgtaactaga
      301 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
      361 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacnaact aaagaattac
      421 naaaacaaat tacnaaaatt cganattttc gggtttatta cagggacagc agagatccac
      481 tttggcgccg gctcgagggg gcccgggtgc aaagatggat aaaagtttta aacanagagg
      541 aatctttgca gctaatggac cttctangtc
//
