LOCUS       sequence-188667-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PKN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PKN2 gRNA (BRDN0001149537)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        168..243
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    299..416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
       61 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
      121 atatcttgtg gaaaggacga aacaccgttg ttgctagtag aacaacggtt ttagagctag
      181 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      241 tgctttttta agcttggcgt aactagatct tgagacaaat ggcagtattc atccacaatt
      301 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
      361 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
      421 tttattacag ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa
      481 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga
      541 aaggagtggg aattggctcc ggtgcccgtc
//
