LOCUS       sequence-188669-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PKN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PKN2 gRNA (BRDN0001487065)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     70..89
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        169..244
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    300..417
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
       61 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
      121 tatatcttgt ggaaaggacg aaacaccgtt tgaagctcga taatactggt tttagagcta
      181 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      241 gtgctttttt aagcttggcg taactagatc ttgagacaaa tggcagtatt catccacaat
      301 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
      361 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
      421 gtttattaca gggacagcag agatccactt tggcgccggc tcgagggggc ccgggtgcaa
      481 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
      541 aaaggagtgg gaattggctc cggtgcccgt
//
