LOCUS       sequence-188703-         485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human ADRBK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: ADRBK2 gRNA (BRDN0001147892)
FEATURES             Location/Qualifiers
     source          1..485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        167..242
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aannnnnnga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
       61 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      121 tatcttgtgg aaaggacgaa acaccgtatt ggacgaggag gattcggttt tagagctaga
      181 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      241 gcttttttaa tcttggcgta actagatctt ganacaaatg gcagtattca tccacaattt
      301 taaaagaaaa ggggggattg gggggtacag tgcaggggan agaatagtan acataatacc
      361 aacagacata cnanctaaag aattacaaaa acaaattaca aaanttgnaa attttcnggt
      421 ttattacnng gacagcnnag atccactttg gcnccggctc gagggggccc gggtgcaaag
      481 atgga
//
