LOCUS       sequence-188769-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PGK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PGK1 gRNA (BRDN0001144730)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..86
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        166..241
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    297..414
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 antannngac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
       61 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      121 atcttgtgga aaggacgaaa caccggctca taaggactac cgactgtttt agagctagaa
      181 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      241 cttttttaag cttggcgtaa ctagatcttg agacaaatgg cagtattcat ccacaatttt
      301 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      361 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      421 tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg ggtgcaaaga
      481 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
      541 ggagtgggaa ttggctccgg tgcccgtcag
//
