LOCUS       sequence-188771-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PGK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: PGK1 gRNA (BRDN0001487049)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        163..238
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    294..411
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
       61 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      121 ttgtggaaag gacgaaacac cgtaaggtgc tcaacaacat gggttttaga gctagaaata
      181 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      241 ttttaagctt ggcgtaacta gatcttgaga caaatggcag tattcatcca caattttaaa
      301 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
      361 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
      421 tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggt gcaaagatgg
      481 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
      541 gtgggaattg gctccggtgc ccgtcagtgg
//
