LOCUS       sequence-188779-         491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human BRD2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: BRD2 gRNA (BRDN0001148966)
FEATURES             Location/Qualifiers
     source          1..491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     77..96
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        176..251
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ttagtacnan nnatnngacg gtagaaagta ataatttctt gggtagtttg cagttttaaa
       61 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
      121 ggctttatat atcttgtgga aaggacgaaa caccgaccac tctctctacg cataggtttt
      181 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
      241 cgagtcggtg cttttttaag cttggcgtaa ctagatcttg agacaaatgg cantattcat
      301 cnncaatttt aaaagaaaag gggggattgg ggggtacagt gcatgggaaa gaatattaga
      361 cataatagca acagacatac naactaanga attacaaaaa caaattacaa taattcgaaa
      421 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg
      481 ggtgcaagat g
//
