LOCUS       sequence-18880-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Tiscornia G, Tergaonkar V, Galimi F, Verma IM
  TITLE     CRE recombinase-inducible RNA interference mediated by lentiviral 
            vectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 May 11. 101(19):7347-51.
  PUBMED    15123829
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 May 11. 101(19):7347-51."
COMMENT     Sequence Label: PGTE+mU6-WT
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        47..358
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     278..298
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     regulatory      390..399
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      394..403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(433..452)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(434..452)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(459..476)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(459..475)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(468..490)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(703..722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tggtcgacct gcaggcggcc gcgaattcac tagtgattcc caagttatcc gacgccgcca
       61 tctctaggcc cgcgccggcc ccctcgcaca gacttgtggg agaagctcgg ctactcccct
      121 gccccggtta atttgcatat aatatttcct agtaactata gaggcttaat gtgcgataaa
      181 agacagataa tctgttcttt ttaatactag ctacatttta catgataggc ttggatttct
      241 ataagagata caaatactaa attattattt taaaaaacag cacaaaagga aactcaccct
      301 aactgtaaag tagttgtgtg ttttgagact ataaatatcc ctggagaaaa gcctgtttgt
      361 ggtcttcgga tcccaatcga attcccgcgg ccgccatggc ggccgggagc atgcgacgtc
      421 gggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
      481 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      541 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      601 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      661 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      721 cttcctttct cgccac
//
