LOCUS       sequence-18884-e         519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Miyoshi H, Blomer U, Takahashi M, Gage FH, Verma IM
  TITLE     Development of a self-inactivating lentivirus vector.
  JOURNAL   J Virol. 1998 Oct . 72(10):8150-7.
  PUBMED    9733856
REFERENCE   2  (bases 1 to 519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1998 Oct . 72(10):8150-7."
COMMENT     Sequence Label: pCS-G
FEATURES             Location/Qualifiers
     source          1..519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(304..348)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgac cggtagcaat tcctgcagcc cgggggatcc
       61 gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc
      121 gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
      181 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
      241 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
      301 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
      361 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
      421 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcaat
      481 ccaaggcaca gcagtggtgc aaatgagttt tccagagca
//
