LOCUS       sequence-188861-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human CKM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: CKM gRNA (BRDN0001148653)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgctagaaag taataatttc ttgggtagtt tgcngtttta aaattatgtt ttaaaangga
       61 ctancatatg cttaccgtaa cttgaaagta tttcgattnc ttggctttat atatctgggg
      121 gaaaggacga aacnccggta gacgatgtca tccagacgtt ttaaagctag aaatancaag
      181 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc nccgagccng tgctttttta
      241 ancttgncgt aactanatct ngagacaaat ggccgtnttc ctccacaatt tnaaaanana
      301 aggggggatt ggggggtaca gngcngggga aagaatanta nacataatan cancngacat
      361 ncannctaaa gaattacnaa aacanantnc aagagattca aaattttcgg gtttantaca
      421 gggncngcag agcatccact ttggcgccgg ctcggggggg cccgggtgca nagagggata
      481 gacgccttna aacanaggag gaanctttgc nnctnatggn cctgncnatn ntcttganag
      541 ggagnggggc anttggntcc ggtgccncnt
//
