LOCUS       sequence-188909-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human DCK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: DCK gRNA (BRDN0001148859)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        160..235
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    291..408
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
       61 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      121 tggaaaggac gaaacaccgt caagaaaatc tccatcgaag ttttagagct agaaatagca
      181 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      241 taagcttggc gtaactagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
      301 aaagggggga ttggggggta cagtgcangg gaaagaatag tagacataat agcaacagac
      361 atacaaacta aagaattaca aaaacaaatt acnaaaattc aaaattttcg ggtttattac
      421 agggacagca gacatccact ttggcgccgg ctcgaggggg cccgggtgca nagatggata
      481 aagttttaac cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
      541 ggaattggct ccggtgcccg tcagtgggca
//
