LOCUS       sequence-18893-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   DeLaBarre B, Christianson JC, Kopito RR, Brunger AT
  TITLE     Central pore residues mediate the p97/VCP activity required for 
            ERAD.
  JOURNAL   Mol Cell. 2006 May 19. 22(4):451-62.
  PUBMED    16713576
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 May 19. 22(4):451-62."
COMMENT     Sequence Label: pBD-0069
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tgaatatttt gtttacttta gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag ccatatggcc tctggagccg attcaaaagg
      121 tgatgattta tcaacagcca ttctcaaaca gaagaaccga cccaatcggt taattgttga
      181 tgaagccatc aatgaagata acagcgtggt gtccttgtcc cagcccaaga tggatgaact
      241 gcagttgttc cgaggtgaca cggtgttgct aaaaggaaag aaaagacggg aagctgtatg
      301 cattgttctt tctgatgaca cgtgttctga tgagaagatt cgaatgaata gagttgttcg
      361 gaataacctc cgagttcgcc taggagatgt catcagcatc cagccatgcc ctgatgtaaa
      421 gtatggcaaa cgtatccacg ttctacccat cgatgacaca gtggaaggca tcactggcaa
      481 tctctttgag gtatacctta agccgtactt cctggaagct tatcggccca tccgtaaagg
      541 agatattttt cttgtccggg gtgggatgcg tgctgtggag ttcaaagttg tagagacaga
      601 tcccagccct tactgtattg ttgctccaga cacagtgatc cactgtgagg gggagccaat
      661 caaacgagag gatgaggagg aatccttgaa tgaagtaggc tatgatgacg tcggtggttg
      721 caggaagcag ctagctcaga taaaggagat ggtggagctg ccactgagac atcctgcgct
      781 c
//
