LOCUS       sequence-18905-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  TITLE     Bjorkman lab plasmids
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGP67A - murine TfR
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     117..176
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             186..203
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             213..224
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tataaaaaaa cctataaata tctccggatt attcataccg tcccaccatc gggcgcggat
       61 ctatgctact agtaaatcag tcacaccaag gcttcaataa ggaacacaca agcaagatgg
      121 taagcgctat tgttttatat gtgcttttgg cggcggcggc gcattctgcc tttgcggcgg
      181 atccgcatca tcatcatcat cacagcagcg gcatcgaggg acgtggtgaa ttccgcttat
      241 attgggcaga cctcaaaaca ctgttgtcag agaagttgaa ctccatagag tttgctgaca
      301 ccatcaagca gctgagccag aatacataca ctcctcgtga ggctggatct caaaaagatg
      361 aaagtcttgc ctattatatt gaaaatcagt tccatgaatt taaattcagc aaagtctggc
      421 gagatgaaca ctatgtgaag attcaagtga aaagcagcat tggtcaaaac atggtgacca
      481 tagtgcagtc aaatggtaac ttagacccag tggagtctcc cgagggttat gtggcattca
      541 gtaaacctac agaagtttct ggtaaactgg tccatgctaa ttttggcact aaaaaggact
      601 ttgaagaact aagttattct gtgaatggat ctttagtgat tgttagagca ggggaaatta
      661 cttttgcaga anaggttgca aatgccccaa gctttaatgc aattggtgtc ctcatataca
      721 tggacaagaa taaattcccc gttgttgagg cagaccttgc actctttgga catgctcatc
      781 t
//
