LOCUS       sequence-189138-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral gRNA plasmid, non-targeting control gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: non-targeting control gRNA (BRDN0001146467)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgctagaaag taataatttc ntgggnagtt tgcagctttt aaaattatgt tttaaaatgg
       61 actatcatat gcttaccgct aacttgaaag tatttcgatt tcttggcttt atatatcttg
      121 tgganaggac gaaacaccgc cagtgccctt ttgtcgcaag ttttatagct ngaaatagca
      181 agttaaaata aggcnagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      241 taagcttggc gtanctagat cttgagacaa atggnagtat tcatccacna ttttaaaana
      301 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat ancaacagac
      361 atacnaacta angaattacn anaacaantt acaaaaattc aanattttcg ggtttattac
      421 agggacagca gagatccact ttgncgccgg ctcgaggggg cccgggtgca aagatggata
      481 aagttttaan cagacaggaa tctttgcagc naatggacct tctaggtctt gaaaggagtg
      541 ggaattggct ccngngcccg tcngtgggca
//
