LOCUS       sequence-189181-        9570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with 
            Dam-V5 using Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label:  pLgw NKX2-5Y191C-V5-EcoDam
FEATURES             Location/Qualifiers
     source          1..9570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..225
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      271..699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(358..377)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     568..589
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(708..728)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        713..1042
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      893..1028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     955..974
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1090..1137
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1156..1530
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1660..1781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1697..1716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1751..1770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1830..1846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1830..1846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1843..1865)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1854..1870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1878..1884,1885..1902,1903..1908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1923..1944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2061..2078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2232..2820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2312..2331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2991..3782,3783..3851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3614..3633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3852..3956)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4031..4050)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4222..4601
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4603..4801
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4756..4776
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4819..4999
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5046..5171
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5664..5897
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6082..6126
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6588..6826
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6773..6794
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6879..6903
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7918..7942)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7953..7994
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     LTR             9359..9539
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(join(9565..9570,1..12))
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
       61 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      121 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
      181 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
      241 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
      301 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      361 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      421 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      481 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      541 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      601 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
      661 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
      721 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      781 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      841 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
      901 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
      961 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     1021 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     1081 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     1141 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     1201 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     1261 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     1321 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     1381 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     1441 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     1501 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     1561 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     1621 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     1681 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1741 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     1801 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1861 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1921 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1981 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2041 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2641 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3301 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3601 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3961 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     4021 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     4081 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     4141 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     4201 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     4261 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     4321 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     4381 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     4441 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     4501 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     4561 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     4621 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     4681 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     4741 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     4801 gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4861 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4921 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4981 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     5041 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     5101 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     5161 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     5221 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     5281 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     5341 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     5401 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     5461 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     5521 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     5581 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     5641 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     5701 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     5761 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     5821 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     5881 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     5941 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     6001 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     6061 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     6121 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     6181 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     6241 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     6301 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     6361 ttagtgaacg gatcccccgg gctgcaggaa ttcagatctc ggccgcatat taagtgcatt
     6421 gttctcgata ccgctaagtg cattgttctc gttagctcga tggacaagtg cattgttctc
     6481 ttgctgaaag ctcgatggac aagtgcattg ttctcttgct gaaagctcga tggacaagtg
     6541 cattgttctc ttgctgaaag ctcagtaccc gggagtaccc tcgaccgccg gagtataaat
     6601 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     6661 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag
     6721 tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac
     6781 tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata ctttcaacaa
     6841 gttaccgaga aagaagaact cacacacagc tagatcaaac aagtttgtac aaaaaagcag
     6901 gctggcgccg gaaccaattc agtcgactgg atccgaccat gttccccagc cctgcgctca
     6961 cacccacgcc tttctcagtc aaagacatcc tgaacctgga gcagcagcag cgtagcctgg
     7021 cgtctgggga cctgtctgcg cgcctcgagg ccaccctggc ccctgcctcc tgcatgctgg
     7081 ccgccttcaa gcccgaggcc tactctggcc ccgaggcggc agcgtccggc ctggcagagc
     7141 tgcgcgcgga gatgggcccc gcgccttcgc cccccaagtg ctctcctgct ttcccagccg
     7201 cccccacatt ttacccggga gcctacggtg accctgaccc agccaaagac cctcgggcgg
     7261 ataaaaaaga gctgtgcgcg ctgcagaagg cagtggagct ggacaaagcc gagacggatg
     7321 gcgccgagag accacgcgca cggcggcgac ggaagccacg cgtgctcttc tcgcaggcgc
     7381 aggtctacga gctggagcgg cgcttcaagc aacagcggta cctgtcggcg ccagagcgcg
     7441 accagctggc cagcgtgctg aagctcacgt ccacgcaggt caagatctgg ttccagaacc
     7501 gtcgctgcaa gtgcaagcga cagcggcagg accagactct ggagcttctg gggccgccgc
     7561 cgccgcccgc gcgcaggatc gcggtgcccg tgctggtgcg cgacgggaag ccctgcctgg
     7621 gggaccccgc ggcctacgct cccgcctacg gcgtgggtct caatgcctat ggctacaacg
     7681 cctaccccta ccccagctac ggcggcgcgg cctgcagtcc cggctacagc tgcgccgcct
     7741 accccgctgc gccccccgcc gcgcagcccc ccgccgcctc cgccaacagc aacttcgtga
     7801 actttggcgt cggggacttg aacaccgtgc agagtcccgg gatgccgcag ggcaattcgg
     7861 gcgtctccac gctgcacggc atccgagcct ggtcgcggcc gcactcgaga tatctagacc
     7921 cagctttctt gtacaaagtg gttcgatccg gtggtaagcc tatccctaac cctctcctcg
     7981 gtctcgattc tacgatcgat ttcgaaccgc ggatgaagaa aaatcgcgct tttttgaagt
     8041 gggcaggggg caagtatccc ctgcttgatg atattaaacg gcatttgccc aagggcgaat
     8101 gtctggttga gccttttgta ggtgccgggt cggtgtttct caacaccgac ttttctcgtt
     8161 atatccttgc cgatatcaat agcgacctga tcagtctcta taacattgtg aagatgcgta
     8221 ctgatgagta cgtacaggcc gcacgcgagc tgtttgttcc cgaaacaaat tgcgccgagg
     8281 tttactatca gttccgcgaa gagttcaaca aaagccagga tccgttccgt cgggcggtac
     8341 tgtttttata tttgaaccgc tacggttaca acggcctgtg tcgttacaat ctgcgcggtg
     8401 agtttaacgt gccgttcggc cgctacaaaa aaccctattt cccggaagca gagttgtatc
     8461 acttcgctga aaaagcgcag aatgcctttt tctattgtga gtcttacgcc gatagcatgg
     8521 cgcgcgcaga tgatgcatcc gtcgtctatt gcgatccgcc ttatgcaccg ctgtctgcga
     8581 ccgccaactt tacggcgtat cacacaaaca gttttacgct tgaacaacaa gcgcatctgg
     8641 cggagatcgc cgaaggtctg gttgagcgcc atattccagt gctgatctcc aatcacgata
     8701 cgatgttaac gcgtgagtgg tatcagcgcg caaaattgca tgtcgtcaaa gttcgacgca
     8761 gtataagcag caacggcggc acacgtaaaa aggtggacga actgctggct ttgtacaaac
     8821 caggagtcgt ttcacccgcg aaaaaataac tcgagaccta gaaaaacatg gagcaatcac
     8881 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     8941 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     9001 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     9061 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     9121 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     9181 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     9241 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     9301 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     9361 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9421 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9481 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9541 ggcccgttta aacccgctga tcagcctcga
//
